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Luigi Buscalioni
After Cavara transferred to Naples in 1906, he was succeeded by Luigi Buscalioni (1863-1954), known in Italy and abroad for his scientific work in many different fields of botany; furthermore he was occupied with the enlargement of the Institute, particularly with the creation of space for the library and laboratories.

The new buildings, nevertheless, were very modest from an architectural point of view when compared to Tornabene's classic building.

Buscalioni attempted to create Englerís systematic order in the garden, but the substantial modifications, although allowing a more rationalised organisation, led to the destruction of trees of doubtless value.